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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effect of Combinations of Cryoprotectants and Freezing Rates on Cryopreservation of the Spermatozoa of Striped Catfish, Pangasianodon hypophthalmus (Sauvage, 1878)) ผู้เขียน: Samorn Ponchunchoovong, Sunai Plime สื่อสิ่งพิมพ์:pdf AbstractTwo experiments were carried out involving sperm cryopreservation of striped catfish, Pangasianodon hypophthalmus,. In the first experiment, the effects of combinations of cryoprotectants (dimethyl sulfoxide (DMSO 10%), dimethyl acetamide (DMA 10%) and methanol (5%)) were investigated. The combination of cryoprotectants involving DMSO (10%) + DMA (20%) had the highest fertilization rate of 59% (99% of control), followed by DMA (20%) + MeOH (5%) and DMSO (10%) + DMA (10%). These results were not significantly (p>0.05) different from the control. The percentage among treatments of viability and motility was lower than that of the control treatment (p<0.05). In the second experiment, each combination of cryoprotectants from the first experiment that had produced excellent fertilization rates (10% DMSO + 10% DMA, 10% DMSO + 20% DMA and 20% DMA + 5% MeOH) was mixed with 0.9% NaCl as extender and the effects of four freezing rates at 5, 10, 20 and 40?C min-1 on the cryopreservation of striped catfish were investigated. The highest fertilization rate was 75% (98% of control) resulting from 5?C min-1 using the combination of DMSO and DMA (10%). The freezing rates of 5 and 10?C min-1 yielded a similar fertilization percentage with the control (p>0.05). The outcomes from this study indicated that the combination of cryoprotectants used (5% MeOH and 20% DMA) increased the fertilization percentage compared with using 5% MeOH alone and also indicated that increasing the freezing rate up to 40?C min-1 decreased the fertilization rate of striped catfish sperm. |
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